Our cutting-edge analytical solutions, combining CryoTEM with our in-house image analysis software, enables to accurately assesses liposomes and drug delivery characteristics through a complete portfolio of solutions such as overall morphology, lamellarity, membrane thickness, packaging, and circularity. 

Packaging asseessment

Our cryo-TEM analyses offer a detailed view of the packaging of your liposomes, providing valuable insights into their encapsulation efficiency. This data is crucial for evaluating the performance of drug delivery systems and is delivered conveniently to you in a comprehensive analytical report.

Figure 1. filled and empty

CryoTEM preparation and imaging allows to observe the specimen and its particles in a near-native hydrated state. Through this method, the inner content of the particles can be directly visualized and assessed, allowing a straight forward discrimination between particles rich in drug and empty ones.

Figure 2_Packaging analysis

Our semi-automated detection method using our in-house software solution presents a representative histogram (left) that effectively displays the liposome packaging. The corresponding image (right) simplifies data interpretation by overlaying detected and classified particles with clear blue/green/red outlines. This allows for a visual, user-friendly understanding of encapsulation efficiency. The percentage of encapsulation can be correlated to the efficiency of the drug release. This data is provided in the analytical report.

Lamellarity Assessment

Insights on the liposomes lamellarity is essential for predicting drug release kinetics. CryoTEM combined with image analysis from our software package allows to distinguish liposomes based on their lamellarity. 

Figure 3. Lamellarity morphology

Our cryo-TEM images display the morphology of liposomes with different lamellarities, illustrating the clear distinction between unilamellar, multilamellar, and multiparticle liposomes. These images provide support for understanding the of role the structural characteristics in drug release kinetics.

Figure 4. Lamellarity

A representative histogram (left) efficiently displays the various lamellarities. The corresponding image (right) brings the data to life by overlaying detected and classified particles with vibrant red/yellow/green outlines, facilitating an easy interpretation of liposome structure. Whether your liposomes are unilamellar, multilamellar, or multiparticle, we provide you with precise and reliable data.

Size and Internal Volume Measurements

In liposome research, size and internal volume are key. Thanks to our AI-powered VAS software used in conjunction with cryo-TEM, you can ensure the highest level of quality control by obtaining reliable measurements of these crucial attributes. Our software provides clear, easy-to-understand visualizations of these measurements, enabling you to maintain top-notch quality control with ease.

Figure 5. size _ volume

Dive into detailed insights with our representative histograms showcasing the size distribution (left) and internal volume (right) of liposomes. These vital metrics, derived from the semi-automated detection of approximately 1,500 particles, provide comprehensive data necessary for your liposome formulation and research optimization. Our technology brings precision and reliability to your fingertips.

Circularity

The circularity of liposomes impacts their behavior in biological systems and their drug delivery efficiency. Our advanced cryoTEM analyses allow you to assess the circularity of your liposomes, providing you with critical data to ensure the safety and efficacy of your formulation.

Figure 6. Circularity

Experience the precision of our VAS software through a representative histogram (left) that displays the circularity of Doxorubicin-containing liposomes. This is complemented by a cryo-TEM image (right), the basis of our analysis, illustrating the intricate details of liposome structure. Our advanced tools provide you with actionable data to guide your research and optimize your liposome formulation.

Maximize Efficiency and Efficacy with Precise Measurement of Lipid Bilayer Thickness

Recognizing the significant impact of lipid bilayer thickness on the efficiency and efficacy of liposomal products, we’ve made it an essential parameter to monitor in the design of liposome-based drug carriers. By harnessing the capabilities of both cryoTEM and our proprietary VAS software, you can measure the thickness of the lipid bilayer with unmatched precision.

Figure 7. membrane thicknessMembrane thickness assessment might be required by some authorities for the release of drug product. They reflect the status of the membrane and give insights on its structure and when relevant, its functionalization. Accurate measurements of the liposomal bilayer thickness can be achieve with our proprietary software, through analysis of higher magnification images where the bilayer can be observed and measured.